The ending of Mass Effect 2 is pretty powerful if you let everybody die who can die. (SPOILER)Your entire team, including Shepard, and the entire Normandy crew save Joker and of course EDI. At the end it's just Joker and Shepard, and Joker's too weak to hold off the swarm AND save Shepard, so he's left all alone. Later you see him in a room full of the crew's caskets.