Many of these require context to really enjoy the joke, but for me, these are the quotes that I actually have used in various conversations.

Kronk: Oh, right. The poison. The poison for Kuzco, the poison chosen especially to kill Kuzco, Kuzco's poison. That poison?

Yzma: Ah, how shall I do it? Oh, I know. I'll turn him into a flea, a harmless, little flea, and then I'll put that flea in a box, and then I'll put that box inside of another box, and then I'll mail that box to myself, and when it arrives... AHAHAHAHAHA, I'll smash it with a hammer!

Kronk: Faster! Faster! Yzma, put your hands in the air!

Yzma: Is that my voice? Is that my voice!? Oh well.

Yzma: Pull the lever, Kronk!
Yzma: Wrong lever!
Yzma: Why do we even have that lever?

Kronk's Shoulder Angel: No, no. He's got a point.

Yzma: Ha! You really should have thought of that before you became peasants!

Old Man: Oh, it's not the first time I was tossed out of a window, and it won't be the last. What can I say? I'm a rebel.

Theme Music Guy: WHAT'S HIS NAME???

Kronk's Shoulder Angel, Kronk's Shoulder Devil, Kronk: That'll work.

Kuzco: So... who's in my chaaaaaair?
Kronk: Oh, oh! I know! Yzma. Yzma's in your chair, right?
Kuzco: Very good, Kronk! Here. Get the snack.

Kronk: Yeah, weird.

Kronk: Well, ya got me. By all accounts, it doesn't make sense.

Kuzco: It's my birthday gift to me. I'm so happy.

Kuzco: D'oh! You threw off my groove!
Guard: I'm sorry, but you've thrown off the Emperor's groove.
Old Man: Sooooorryyyyyy!

Chaca: Yeah-hah!

Tipo: Nuh-uh!

Kuzko: Huh? What? I didn't do anything. I didn't... Somebody's throwin' stuff.

Kuzco: I have no idea. You're the criminal mastermind, not me.

Kuzco: You're right. That's giving you way too much credit.

Kuzco: So, you lied to me.

Kronk: You owe me a new acorn.

Junior Chipmunk Class: Squeak, squeakin', squeak, squeakity.
and other mixed up versions of the squeaky stuff

Multiple people describing Yzma: Scary beyond all reason.

Yzma: Well, he ain't getting any deader!

Kronk: Oh, yeah, it's all comin' together.

Old Man: Bewaaaaaaaare the grooooooooove.

Pacha: Demon llama!

Kuzco: Boom, baby!

Yzma: You think!?

Kuzco: Wahhhhh! Llama face!

ChiCha: I gotta go wash something.

Kuzco: Uh-huh! Uh-huh, uh-huh, uh-huh!

Kuzco: HA! *immediately covers mouth in realisation that shouting "HA!" has suddenly put me in danger*

Yzma: Take it, Kronk. Feel the power!
Kronk: Oh... I can feel it.

Kuzco: Winner...
Kuzco: Loser!

Kuzco: Oh, he's doing his own theme music? Big, dumb and tone deaf.

Kuzco: Booya.

Cow Guard: Hey, I've been turned into a cow. Can I go home?

Kronk: Right. Oh! Riiiiiiggghhht...

Bug in jungle: Help me! Help me!
Bug in jungle: Too late...

Kuzco: Well, whatever you call it, it was disgusting.

Kuzco: No touchy!

Kuzco: Woo-yeah! Look at me and my bad self!

Kuzco: Hit the road, Bucky!

Kuzco: Hmm. Don't know, don't care.

Kuzco: Yay, I'm a llama again! No, wait.

Seriously, there is a TENG quote for every moment. I think I uncovered a few more I might use soon enough while going through the IMDb quotes list.