This is in no particular order, but here's the first.
Samus Aran
Metroid Game Series.
Favorite Appearance to date. Super Metroid (no surprise)
This is the "real" Samus to me. None of this 5'1" crap or whatever she's supposed to be now. She's a no nonsense 6'3" 200lb cybernetically enhanced bruiser, but she's smart and she has morals. I actually didn't mind the approach they took towards her personality in the prequels. She hardened up over the years full of tough jobs in remote settings working alone.
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